Monday, July 25, 2016

Breast cancer: Information and hypotheses.........

Breast tissue is made of adipose cells and lympatic glands..
The suspected gene BRCA1 is on chromosome 17 and BRCA2 is on chromosome 13
ER (estrogen) -positive cancer:
Luminal A cell lines: MCF-7 and T47D
Metastatic: MDA-MB-231

Common endocrine-disrupting agents: aluminum, prabens, triclosan, phthalates, perfumes
#Mammograms test detects tumor in breast tissue. However, its radiation itself is not safety-proof. Accumulated radiation doses can initiate mutation itself.

#Persistent infection and allergen exposure can cause lymph gland to swell. sensing danger, aromataae enzyme iwll overexpress, producing estrogen, which can cause hyperplasia...

#Its a good piece of information to know that some tumor disappear undetected, untreated. That means, if the source of inflammation or abuse is removed, the signalling is likely to correct and immune attack is likely to subside. Proteasome complex might degrade the offensive proteins.

#As prevention is always better than therapy, one must not abuse body through bad lifestyles.
Healthy lifestyle includes simple, minimally-processed balanced food,  lower exposure to chemicals (pesticides, cosmetics, cleaning agents, food additives), physical activity, vitamin D from sun, stress-free life..

Thursday, July 21, 2016

Cancer types and cell lines..........

Cancer is a heterogeneous disease. Above all, therapeutic success in unpredictable.
Its result of inflammation...caused by perturbed proteases, wrecking havoc with normal functionality of body. Pesticides and household endocrine disruptors are increasing risks of cancer.
Personalized medicine is required to treat as genetics of each individual is different.
Poor prognosis, metastasis, high relapse rate make cancer deadly
Mapping the mutations, genes and their pathways can reveal a lot about cancer.
Diagnois: Ultrasound, colonoscopy, mammography..
Current therapeutic strategy include: surgery (mastectomies), chemo, radiotherapy, molecular targeted therapy

TCGA: Cancer Genome Atlas consortium
26L5:  murine colon carcinoma
B16BL6:  murine melanoma,
murine Lewis lung carcinoma
A375 : human melanoma
A498: Renal carcinoma
A549:  human lung adenocarcinoma
AMC-HN-4:  malignant human head and neck
BT474: human breast
ChaGo: human bronchogenic
CNE1:  nasopharyngeal carcinoma
DU145:  hormone-resistant prostate cancer
GBM : human glioblastoma
HCC: Hepatocellular carcinoma
HeLa: human cervix adenocarcinoma
Hep-G2:human liver
HT-1080:  humanfibrosarcoma
KATO-III: human gastric
LNCaP: hormone-sensitive prostate cancer
MCF-7:  human breast cancer ERα+
mCRPC: metastatic castration resistant prostate cancer
PBMC: uman peripheral blood mononuclear cell
PC-3:  human colon carcinoma
SW620: human colon
U87MG:  human glioblastoma
Normal cell lines (control)
HS27: fibroblast
SW480 cells

Human genes associated with different cancers/cancer-associated genes:
Colon: BCL9L, RBM10, CTCF, and KLF5
Cervical adenocarcinoma:
Breast cancer: BRCA1 and BRCA2
Ovarian cancer. BRCA1 and BRCA2
Well-known cancer pathways
Wnt pathway
Wnt binds to its receptor Frizzled, and potential co-receptor LRP-5/6
It suppresses GSK-3ß phosphorylation of ß-Catenin.
ß-Catenin accumulates in nucleus
it binds to LEF/TCF transcription factors, which activate Wnt target genes.
Wnt binds to Dishevelled protein by tyrosine kinase